Comparison of two molecular assays with conventional blood culture for diagnosis of sepsis

Eva Leitner; Harald H Kessler; Walter Spindelboeck; Martin Hoenigl; Csilla Putz-Bankuti; Vanessa Stadlbauer-Köllner; Robert Krause; Andrea J Grisold; Gebhard Feierl; Rudolf E Stauber

doi:10.1016/j.mimet.2012.12.012

Comparison of two molecular assays with conventional blood culture for diagnosis of sepsis

J Microbiol Methods. 2013 Mar;92(3):253-5. doi: 10.1016/j.mimet.2012.12.012. Epub 2012 Dec 22.

Authors

Eva Leitner¹, Harald H Kessler, Walter Spindelboeck, Martin Hoenigl, Csilla Putz-Bankuti, Vanessa Stadlbauer-Köllner, Robert Krause, Andrea J Grisold, Gebhard Feierl, Rudolf E Stauber

Affiliation

¹ Institute of Hygiene, Microbiology and Environmental Medicine (IHME), Medical University of Graz, Austria.

PMID: 23266578
DOI: 10.1016/j.mimet.2012.12.012

Abstract

In this small study, the LightCycler® SeptiFast, and the SepsiTest™ were compared with blood culture. The SeptiFast showed a higher sensitivity (42.9%) and specificity (88.2%) when compared to blood culture than the SepsiTest™ (28.6 and 85.3%). The SeptiFast provides more species specific results, although the identification panel is smaller.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / classification
Bacteria / genetics
Bacteria / isolation & purification*
Bacteriological Techniques / methods*
Humans
Molecular Diagnostic Techniques / methods*
Sensitivity and Specificity
Sepsis / diagnosis*
Sepsis / microbiology*