We determined seroprevalence of antibodies to pandemic influenza H1N1 (2009) virus in outpatients in China from December 2009 to March 2010. Serum antibody titers were determined by a hemagglutination-inhibition (HI) assay using the seroprevalence data for 2006-2008 (1.2%) as baseline. The overall seroprevalence was 7.6%, 18.6%, 20.5%, and 20.0% in December 2009, January 2010, February 2010, and March 2010, respectively. In comparison of monthly data, the seroprevalence values for the first three months exhibited statistically significant differences. As for the age-specific seroprevalence, the individuals aged demic virus.