Polymorphisms in ERCC1 and XPF genes and risk of gastric cancer in an eastern Chinese population

PLoS One. 2012;7(11):e49308. doi: 10.1371/journal.pone.0049308. Epub 2012 Nov 15.

Abstract

Background: Inherited functional single nucleotide polymorphisms (SNPs) in DNA repair genes may alter DNA repair capacity and thus contribute to cancer risk.

Methods: Three ERCC1 functional SNPs (rs2298881C>A, rs3212986C>A and rs11615G>A) and two XPF/ERCC4 functional SNPs (rs2276466C>G and rs6498486A>C) were genotyped for 1125 gastric adenocarcinoma cases and 1196 cancer-free controls by Taqman assays. Odds ratios (OR) and 95% confidence intervals (CI) were used to estimate risk associations, and false-positive report probabilities (FPRP) were calculated for assessing significant findings.

Results: ERCC1 rs2298881C and rs11615A variant genotypes were associated with increased gastric cancer risk (adjusted OR=1.33, 95% CI=1.05-1.67 for rs2298881 AC/CC and adjusted OR=1.23, 95% CI=1.05-1.46 for rs11615 AG/AA, compared with their common genotype AA and GG, respectively). Patients with 2-3 ERCC1 risk genotypes had significant increased risk (adjusted OR=1.56, 95% CI=1.27-1.93), compared with those with 0-1 ERCC1 risk genotypes, and this risk was more significantly in subgroups of never drinkers, non-gastric cardia adenocarcinoma (NGCA) and clinical stage I+II. All these risks were not observed for XPF SNPs.

Conclusions: These findings suggest that functional ERCC1 SNPs may contribute to risk of gastric cancer. Larger and well-designed studies with different ethnic populations are needed to validate our findings.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asian People / genetics*
  • China / epidemiology
  • DNA Repair / genetics
  • DNA-Binding Proteins / genetics*
  • Endonucleases / genetics*
  • Genotype
  • Humans
  • Odds Ratio
  • Polymorphism, Single Nucleotide / genetics
  • Risk Factors
  • Stomach Neoplasms / epidemiology*
  • Stomach Neoplasms / genetics*

Substances

  • DNA-Binding Proteins
  • xeroderma pigmentosum group F protein
  • ERCC1 protein, human
  • Endonucleases

Grants and funding

This research was supported by funds from China’s Thousand Talents Program at Fudan University and the grant from the Ministry of Health (grant number 201002007). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.