Abstract
We found mouse neural progenitor cells (NPCs) to have a secretory protein profile distinct from other brain cells and to modulate microglial activation, proliferation and phagocytosis. NPC-derived vascular endothelial growth factor was necessary and sufficient to exert at least some of these effects in mice. Thus, neural precursor cells may not only be shaped by microglia, but also regulate microglia functions and activity.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Analysis of Variance
-
Animals
-
Animals, Newborn
-
Antigens, CD
-
Antigens, Differentiation, Myelomonocytic
-
Brain / cytology
-
Bromodeoxyuridine / metabolism
-
Calcium-Binding Proteins / metabolism
-
Cell Proliferation / drug effects
-
Cell Transplantation / methods
-
Cells, Cultured
-
Chemotaxis / drug effects
-
Culture Media, Conditioned / pharmacology
-
Dose-Response Relationship, Drug
-
Embryo, Mammalian
-
Gene Expression Regulation / drug effects
-
Glial Fibrillary Acidic Protein / metabolism
-
Green Fluorescent Proteins / genetics
-
Male
-
Mice
-
Mice, Inbred C57BL
-
Mice, Transgenic
-
Microfilament Proteins / metabolism
-
Microglia / drug effects
-
Microglia / physiology*
-
Neural Stem Cells / physiology*
-
Phagocytosis / drug effects
-
Phagocytosis / physiology
-
Proteomics / methods
-
RNA, Small Interfering / metabolism
-
SOXB1 Transcription Factors / metabolism
-
Vascular Endothelial Growth Factor A / metabolism
-
Vascular Endothelial Growth Factor A / pharmacology
Substances
-
Aif1 protein, mouse
-
Antigens, CD
-
Antigens, Differentiation, Myelomonocytic
-
CD68 protein, mouse
-
Calcium-Binding Proteins
-
Culture Media, Conditioned
-
Glial Fibrillary Acidic Protein
-
Microfilament Proteins
-
RNA, Small Interfering
-
SOXB1 Transcription Factors
-
Sox2 protein, mouse
-
Vascular Endothelial Growth Factor A
-
Green Fluorescent Proteins
-
Bromodeoxyuridine