Abstract
Characterization and antitumor activity of basic fibroblast growth factor-mediated active targeting doxorubicin microbubbles (bFGF-DOX-MB) were investigated. Pluronic F68 with chemical conjugation of doxorubicin (DOX-P) and peptide KRTGQYKLC-conjugated DSPE-PEG2000 were prepared. bFGF-DOX-MB had a normal distribution of particle size, with average particle size of 2.7 μm. Using A549 mouse model, bFGF-DOX-MB combined ultrasound showed the best inhibition effect on tumor volume growth among all the test groups. Similar conclusion was obtained from experimental measurements of tumor weight change and blood cell count. From the results, chemotherapeutic drug inhibition on tumor growth could be enhanced by local ultrasound combined with active targeting bFGF-DOX-MB, which might provide a potential application for ultrasound-mediated chemotherapy.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibiotics, Antineoplastic / administration & dosage*
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Antibiotics, Antineoplastic / adverse effects
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Antibiotics, Antineoplastic / chemistry
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Antibiotics, Antineoplastic / therapeutic use
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Cell Line, Tumor
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Doxorubicin / administration & dosage*
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Doxorubicin / adverse effects
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Doxorubicin / chemistry
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Doxorubicin / therapeutic use
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Drug Compounding
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Drug Delivery Systems* / adverse effects
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Feasibility Studies
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Fibroblast Growth Factor 2 / administration & dosage*
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Fibroblast Growth Factor 2 / adverse effects
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Fibroblast Growth Factor 2 / chemistry
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Fibroblast Growth Factor 2 / therapeutic use
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Humans
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Lung Neoplasms / drug therapy*
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Lung Neoplasms / metabolism
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Lung Neoplasms / pathology
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Male
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Mice
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Mice, Nude
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Microbubbles / adverse effects
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Microbubbles / therapeutic use*
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Neoplasm Proteins / metabolism
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Oligopeptides / administration & dosage*
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Oligopeptides / adverse effects
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Oligopeptides / chemistry
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Oligopeptides / therapeutic use
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Peptide Fragments / administration & dosage*
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Peptide Fragments / adverse effects
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Peptide Fragments / chemistry
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Peptide Fragments / therapeutic use
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Protein Interaction Domains and Motifs
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Random Allocation
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Receptor, Fibroblast Growth Factor, Type 2 / metabolism
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Tumor Burden / drug effects
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Xenograft Model Antitumor Assays
Substances
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Antibiotics, Antineoplastic
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Neoplasm Proteins
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Oligopeptides
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Peptide Fragments
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lysyl-arginyl-threonyl-glycyl-glutaminyl-tyrosyl-lysyl-leucyl-cysteine
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Fibroblast Growth Factor 2
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Doxorubicin
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FGFR2 protein, human
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Receptor, Fibroblast Growth Factor, Type 2