Synergistic enhancement of iron oxide nanoparticle and gadolinium for dual-contrast MRI

Biochem Biophys Res Commun. 2012 Sep 7;425(4):886-91. doi: 10.1016/j.bbrc.2012.07.168. Epub 2012 Aug 7.

Abstract

Purpose: The use of MR contrast agents allows accurate diagnosis by exerting an influence on the longitudinal (T(1)) or transverse (T(2)) relaxation time of the surrounding tissue. In this study, we combined the use of iron oxide (IO) particles and nonspecific extracellular gadolinium chelate (Gd) in order to further improve the sensitivity and specificity of lesion detection.

Procedures: With a 7-Tesla scanner, pre-contrasted, IO-enhanced and dual contrast agent enhanced MRIs were performed in phantom, normal animals, and animal models of lymph node tumor metastases and orthotopic brain tumor. For the dual-contrast (DC) MRI, we focused on the evaluation of T(2) weighted DC MRI with IO administered first, then followed by the injection of a bolus of gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA).

Results: Based on the C/N ratios and MRI relaxometry, the synergistic effect of coordinated administration of Gd-DTPA and IO was observed and confirmed in phantom, normal liver and tumor models. At 30 min after administration of Feridex, Gd-DTPA further decreased T(2) relaxation in liver immediately after the injection. Additional administration of Gd-DTPA also immediately increased the signal contrast between tumor and brain parenchyma and maximized the C/N ratio to -4.12±0.71. Dual contrast MRI also enhanced the delineation of tumor borders and small lesions.

Conclusions: DC-MRI will be helpful to improve diagnostic accuracy and decrease the threshold size for lesion detection.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Neoplasms / pathology*
  • Cell Line, Tumor
  • Contrast Media*
  • Female
  • Ferric Compounds*
  • Gadolinium DTPA*
  • Liver / pathology
  • Lymph Nodes / pathology*
  • Lymphatic Metastasis
  • Magnetic Resonance Imaging / methods*
  • Magnetite Nanoparticles*
  • Mice
  • Mice, Inbred BALB C
  • Phantoms, Imaging

Substances

  • Contrast Media
  • Ferric Compounds
  • Magnetite Nanoparticles
  • ferric oxide
  • Gadolinium DTPA