Characterization of a therapeutic model of inhalational anthrax using an increase in body temperature in New Zealand white rabbits as a trigger for treatment

Clin Vaccine Immunol. 2012 Sep;19(9):1517-25. doi: 10.1128/CVI.00292-12. Epub 2012 Jul 25.

Abstract

The development of an appropriate animal therapeutic model is essential to assess the potential efficacy of therapeutics for use in the event of a Bacillus anthracis exposure. We conducted a natural history study that showed New Zealand White rabbits exhibited a significant increase in body temperature (SIBT), changes in hematologic parameters, and increases in C-reactive protein and succumbed to disease with an average time to death of approximately 73 h following aerosol challenge with B. anthracis Ames spores. The SIBT was used as a trigger to treat with a fully human monoclonal antibody directed at protective antigen (PA). Ninety percent (9/10) of the treated rabbits survived the lethal inhalational challenge of B. anthracis. Further characterization investigated the protective window of opportunity for anti-PA antibody administration up to 12 h post-onset of SIBT. Eighty-three percent (5/6) of the rabbits treated at SIBT and 100% (6/6) of those treated at 6 h after SIBT survived challenge. Only 67% (4/6) of the rabbits treated at 12 h after SIBT survived. The increase in body temperature corresponded with both bacteremia and antigenemia (PA in the blood), indicating that SIBT is a suitable trigger to initiate treatment in a therapeutic model of inhalational anthrax.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Anthrax / pathology*
  • Anthrax / therapy*
  • Antibodies, Bacterial / administration & dosage
  • Antibodies, Monoclonal / administration & dosage
  • Antigens, Bacterial
  • Bacillus anthracis / pathogenicity*
  • Bacterial Toxins / antagonists & inhibitors
  • Biomarkers*
  • Blood Cells / physiology
  • C-Reactive Protein / analysis
  • Disease Models, Animal
  • Female
  • Fever / diagnosis*
  • Immunotherapy / methods
  • Male
  • Rabbits
  • Survival Analysis
  • Time Factors

Substances

  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Bacterial Toxins
  • Biomarkers
  • anthrax toxin
  • C-Reactive Protein