Recombinatorial biases and convergent recombination determine interindividual TCRβ sharing in murine thymocytes

J Immunol. 2012 Sep 1;189(5):2404-13. doi: 10.4049/jimmunol.1102087. Epub 2012 Jul 23.

Abstract

Overlap of TCR repertoires among individuals provides the molecular basis for public T cell responses. By deep-sequencing the TCRβ repertoires of CD4+CD8+ thymocytes from three individual mice, we observed that a substantial degree of TCRβ overlap, comprising ∼10-15% of all unique amino acid sequences and ∼5-10% of all unique nucleotide sequences across any two individuals, is already present at this early stage of T cell development. The majority of TCRβ sharing between individual thymocyte repertoires could be attributed to the process of convergent recombination, with additional contributions likely arising from recombinatorial biases; the role of selection during intrathymic development was negligible. These results indicate that the process of TCR gene recombination is the major determinant of clonotype sharing between individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / immunology
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Differentiation / immunology*
  • Female
  • Mice
  • Random Allocation
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Receptors, Antigen, T-Cell, alpha-beta / physiology*
  • Recombination, Genetic / immunology*
  • Thymus Gland / cytology
  • Thymus Gland / immunology*
  • Thymus Gland / metabolism*
  • V(D)J Recombination / immunology*

Substances

  • Receptors, Antigen, T-Cell, alpha-beta