Abstract
MicroRNAs (miRNAs) are a class of 21- to 25-nucleotide non-coding RNAs, some of which are important gene regulators involved in folliculogenesis. In this study, we used CCK-8, real-time PCR and Western blot assays to demonstrate that miR-145 inhibits mouse granulosa cell (mGC) proliferation. Combined with the results of luciferase reporter assays that studied the 3'-untranslated region of ACVRIB mRNA, these assays identified ACVRIB as a direct target of miR-145. The ectopic expression of miR-145 reduced the levels of both ACVRIB mRNA and protein and also interfered with activin-induced Smad2 phosphorylation. Altogether, this study revealed that miR-145 suppresses mGC proliferation by targeting ACVRIB.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Activin Receptors, Type I / genetics*
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Activin Receptors, Type I / metabolism*
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Activins / metabolism
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Activins / pharmacology
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Animals
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Base Sequence
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Cell Proliferation / drug effects
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Cells, Cultured
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Cyclin D2 / genetics
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Cyclin D2 / metabolism
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Female
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Granulosa Cells / cytology*
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Granulosa Cells / drug effects
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Granulosa Cells / metabolism*
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HEK293 Cells
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Humans
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Mice
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Mice, Inbred ICR
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MicroRNAs / genetics*
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MicroRNAs / metabolism*
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Phosphorylation
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Signal Transduction
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Smad2 Protein / metabolism
Substances
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3' Untranslated Regions
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Ccnd2 protein, mouse
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Cyclin D2
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MIRN145a microRNA, mouse
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MicroRNAs
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RNA, Messenger
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Smad2 Protein
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Smad2 protein, mouse
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activin A
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Activins
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Activin Receptors, Type I
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Acvr1b protein, mouse