Sensitive fluorescence biosensor for folate receptor based on terminal protection of small-molecule-linked DNA

Xiaofeng Wei; Wenlie Lin; Na Ma; Fang Luo; Zhenyu Lin; Longhua Guo; Bin Qiu; Guonan Chen

doi:10.1039/c2cc31979a

Sensitive fluorescence biosensor for folate receptor based on terminal protection of small-molecule-linked DNA

Chem Commun (Camb). 2012 Jun 21;48(49):6184-6. doi: 10.1039/c2cc31979a. Epub 2012 May 16.

Authors

Xiaofeng Wei¹, Wenlie Lin, Na Ma, Fang Luo, Zhenyu Lin, Longhua Guo, Bin Qiu, Guonan Chen

Affiliation

¹ Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University), Department of Chemistry, Fuzhou University, Fuzhou, 350002, China.

PMID: 22590712
DOI: 10.1039/c2cc31979a

Abstract

Based on the specific folic acid-folate receptor (FA-FR) interaction, macromolecular FR can bind with FA-linked DNA-small molecule chimeras, which can prevent enzymolysis by exonuclease III (Exo III), enabling a novel fluorescence biosensor for FR to be developed using quinaldine red as a G-quadruplex-binding probe.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques*
DNA / chemistry*
DNA / metabolism
Exodeoxyribonucleases / metabolism
Fluorescence*
Folate Receptors, GPI-Anchored / analysis*
Folate Receptors, GPI-Anchored / chemistry*
Folate Receptors, GPI-Anchored / metabolism
Folic Acid / chemistry
Folic Acid / metabolism
Models, Molecular
Molecular Weight

Substances

Folate Receptors, GPI-Anchored
DNA
Folic Acid
Exodeoxyribonucleases
exodeoxyribonuclease III