Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory clinical specimens using a rapid real-time polymerase chain reaction assay

Maureen H Diaz; Jonas M Winchell

doi:10.1016/j.diagmicrobio.2012.03.024

Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory clinical specimens using a rapid real-time polymerase chain reaction assay

Diagn Microbiol Infect Dis. 2012 Jul;73(3):278-80. doi: 10.1016/j.diagmicrobio.2012.03.024. Epub 2012 Apr 25.

Authors

Maureen H Diaz¹, Jonas M Winchell

Affiliation

¹ Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.

PMID: 22541789
DOI: 10.1016/j.diagmicrobio.2012.03.024

Abstract

We developed a rapid real-time polymerase chain reaction assay for detecting Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory specimens. This procedure provides over 5 times faster results compared to existing methods while maintaining equivalent detection rates for specimens containing limited target organisms.

Published by Elsevier Inc.

Publication types

Evaluation Study

MeSH terms

Bacteriological Techniques / methods*
Chlamydophila Infections / diagnosis
Chlamydophila Infections / microbiology*
Chlamydophila pneumoniae / genetics
Chlamydophila pneumoniae / isolation & purification*
Exudates and Transudates / microbiology*
Humans
Molecular Diagnostic Techniques / methods
Mycoplasma pneumoniae / genetics
Mycoplasma pneumoniae / isolation & purification*
Pneumonia, Mycoplasma / diagnosis
Pneumonia, Mycoplasma / microbiology*
Real-Time Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Time Factors