Chemical reporters for monitoring RNA synthesis and poly(A) tail dynamics

Chembiochem. 2012 May 29;13(8):1112-5. doi: 10.1002/cbic.201200091. Epub 2012 Apr 19.

Abstract

A versatile "clickable" nucleoside: Metabolic labeling of cells is useful in studying the dynamics of biological molecules. N(6) pA can be utilized by all three mammalian RNA polymerases, as well as poly(A) polymerase. Because of its alkyne modification, RNA labeled with N(6) pA can be visualized and purified by using click chemistry.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / analogs & derivatives
  • Adenosine / chemistry
  • Adenosine / genetics
  • Adenosine / metabolism
  • Click Chemistry
  • DNA-Directed RNA Polymerases / chemistry
  • DNA-Directed RNA Polymerases / genetics
  • DNA-Directed RNA Polymerases / metabolism
  • Gene Expression
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Poly A / chemistry*
  • Poly A / genetics
  • Poly A / metabolism
  • RNA / chemical synthesis
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism
  • RNA Polymerase II / metabolism*
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism

Substances

  • RNA, Messenger
  • Poly A
  • RNA
  • RNA Polymerase II
  • DNA-Directed RNA Polymerases
  • Adenosine