The utility of immunohistochemistry for the identification of hematopoietic and lymphoid cells in normal tissues and interpretation of proliferative and inflammatory lesions of mice and rats

Toxicol Pathol. 2012;40(2):345-74. doi: 10.1177/0192623311430695.

Abstract

Expression of antigens in cells and tissues can be readily studied immunohistochemically with the use of antibodies. A panel of antibodies to cell-specific markers can be used to diagnose lesions, including tumors, in the hematopoietic and lymphoid systems. This review discusses the use of readily available antibodies and procedures to identify antigens expressed in normal tissues and in proliferative and inflammatory lesions in formalin-fixed, paraffin-embedded (FFPE) murine specimens.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Antibodies
  • Biomarkers / analysis*
  • Immune System Diseases / diagnosis
  • Immune System Diseases / immunology
  • Immune System Diseases / metabolism
  • Immunohistochemistry / methods*
  • Inflammation / diagnosis
  • Inflammation / immunology
  • Inflammation / metabolism
  • Lymphocytes / cytology*
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Lymphoproliferative Disorders / immunology*
  • Lymphoproliferative Disorders / metabolism
  • Lymphoproliferative Disorders / pathology
  • Mice
  • Myeloid Cells / cytology*
  • Myeloid Cells / immunology
  • Myeloid Cells / metabolism
  • Rats

Substances

  • Antibodies
  • Biomarkers