Quantitative modeling of the terminal differentiation of B cells and mechanisms of lymphomagenesis

Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2672-7. doi: 10.1073/pnas.1113019109. Epub 2012 Jan 30.

Abstract

Mature B-cell exit from germinal centers is controlled by a transcriptional regulatory module that integrates antigen and T-cell signals and, ultimately, leads to terminal differentiation into memory B cells or plasma cells. Despite a compact structure, the module dynamics are highly complex because of the presence of several feedback loops and self-regulatory interactions, and understanding its dysregulation, frequently associated with lymphomagenesis, requires robust dynamical modeling techniques. We present a quantitative kinetic model of three key gene regulators, BCL6, IRF4, and BLIMP, and use gene expression profile data from mature human B cells to determine appropriate model parameters. The model predicts the existence of two different hysteresis cycles that direct B cells through an irreversible transition toward a differentiated cellular state. By synthetically perturbing the interactions in this network, we can elucidate known mechanisms of lymphomagenesis and suggest candidate tumorigenic alterations, indicating that the model is a valuable quantitative tool to simulate B-cell exit from the germinal center under a variety of physiological and pathological conditions.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • B-Lymphocytes / cytology*
  • B-Lymphocytes / immunology
  • Cell Differentiation*
  • Gene Expression Profiling
  • Humans
  • Immunologic Memory
  • Lymphoma / genetics
  • Lymphoma / pathology*