Cytoplasmic dynein moves through uncoordinated stepping of the AAA+ ring domains

Science. 2012 Jan 13;335(6065):221-5. doi: 10.1126/science.1215804. Epub 2011 Dec 8.

Abstract

Cytoplasmic dynein is a homodimeric AAA+ motor that transports a multitude of cargos toward the microtubule minus end. How the two catalytic head domains interact and move relative to each other during processive movement is unclear. Here, we tracked the relative positions of both heads with nanometer precision and directly observed the heads moving independently along the microtubule. The heads remained widely separated, and their stepping behavior varied as a function of interhead separation. One active head was sufficient for processive movement, and an active head could drag an inactive partner head forward. Thus, dynein moves processively without interhead coordination, a mechanism fundamentally distinct from the hand-over-hand stepping of kinesin and myosin.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Cytoplasm / metabolism*
  • Dyneins / chemistry*
  • Dyneins / metabolism*
  • Microtubules / metabolism*
  • Models, Biological
  • Models, Molecular
  • Protein Multimerization
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae Proteins / chemistry*
  • Saccharomyces cerevisiae Proteins / metabolism*

Substances

  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae Proteins
  • Adenosine Triphosphate
  • Dyneins