Hazara virus infection is lethal for adult type I interferon receptor-knockout mice and may act as a surrogate for infection with the human-pathogenic Crimean-Congo hemorrhagic fever virus

J Gen Virol. 2012 Mar;93(Pt 3):560-564. doi: 10.1099/vir.0.038455-0. Epub 2011 Nov 16.

Abstract

Hazara virus (HAZV) is closely related to the Crimean-Congo hemorrhagic fever virus (CCHFV). HAZV has not been reported to cause human disease; work with infectious material can be carried out at containment level (CL)-2. By contrast, CCHFV causes a haemorrhagic fever in humans and requires CL-4 facilities. A disease model of HAZV infection in mice deficient in the type I interferon receptor is reported in this study. Dose-response effects were seen with higher doses, resulting in a shorter time to death and earlier detection of viral loads in organs. The lowest dose of 10 p.f.u. was still lethal in over 50 % of the mice. Histopathological findings were identified in the liver, spleen and lymph nodes, with changes similar to a recent mouse model of CCHFV infection. The findings demonstrate that inoculation of mice with HAZV may act as a useful surrogate model for the testing of antiviral agents against CCHFV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Structures / pathology
  • Animal Structures / virology
  • Animals
  • Disease Models, Animal*
  • Gene Deletion
  • Hemorrhagic Fever Virus, Crimean-Congo / immunology*
  • Hemorrhagic Fever Virus, Crimean-Congo / pathogenicity*
  • Hemorrhagic Fever, Crimean / immunology
  • Hemorrhagic Fever, Crimean / pathology*
  • Hemorrhagic Fever, Crimean / virology*
  • Histocytochemistry
  • Humans
  • Liver / pathology
  • Liver / virology
  • Lymph Nodes / pathology
  • Lymph Nodes / virology
  • Mice
  • Mice, Knockout
  • Receptor, Interferon alpha-beta / deficiency
  • Receptor, Interferon alpha-beta / immunology*
  • Spleen / pathology
  • Spleen / virology
  • Survival Analysis
  • Viral Load

Substances

  • Receptor, Interferon alpha-beta