Cutting edge: Calcium/Calmodulin-dependent protein kinase type IV is essential for mesangial cell proliferation and lupus nephritis

J Immunol. 2011 Dec 1;187(11):5500-4. doi: 10.4049/jimmunol.1102357. Epub 2011 Oct 26.

Abstract

Renal involvement in systemic lupus erythematosus remains a major cause of morbidity and mortality. Although immune parameters that instigate renal damage have been characterized, their link to local processes, which execute tissue damage, is poorly understood. Using genetic-deletion and pharmacological-inhibition approaches, we demonstrated that calcium/calmodulin-dependent protein kinase type IV, which contributes to altered cytokine production in systemic lupus erythematosus patients, controls spontaneous and platelet-derived growth factor-stimulated mesangial cell proliferation and promotes IL-6 production through AP-1. Our studies identified calcium/calmodulin-dependent protein kinase type IV as a valuable treatment target for lupus nephritis and point out the importance of local kidney factors in the expression of tissue damage that, if properly targeted, should enhance clinical benefit and limit toxicity.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blotting, Western
  • Calcium-Calmodulin-Dependent Protein Kinase Type 4 / metabolism*
  • Cell Proliferation*
  • Electrophoretic Mobility Shift Assay
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Interleukin-6 / biosynthesis
  • Interleukin-6 / immunology
  • Lupus Nephritis / enzymology*
  • Lupus Nephritis / pathology*
  • Mesangial Cells / enzymology*
  • Mesangial Cells / pathology*
  • Mice
  • Mice, Inbred MRL lpr
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Interleukin-6
  • Calcium-Calmodulin-Dependent Protein Kinase Type 4
  • Camk4 protein, mouse