Genetic variations and interactions in anti-inflammatory cytokine pathway genes in the outcome of leprosy: a study conducted on a MassARRAY platform

J Infect Dis. 2011 Oct 15;204(8):1264-73. doi: 10.1093/infdis/jir516.

Abstract

Background: Mycobacterium leprae is the etiologic pathogen that causes leprosy. The outcome of disease is dependent on the host genetic background.

Methods: We investigated the association of 51 single-nucelotide polymorphisms (SNPs) in anti-inflammatory cytokines (IL-10, TGFB1, IL-6, IL-4, and IL-13) and receptors (IL-10RA, IL-10RB, TGFBR1, TGFBR2, IL-6R, IL-4R, IL-5RA, IL-5RB, and IL-13RA1) with susceptibility to leprosy in a case-control study from New Delhi in northern India. This was followed by replication testing of associated SNPs in a geographically distinct and unrelated population from Orissa in eastern India. The functional potential of SNPs was established with in vitro reporter assays.

Results: Significant associations (P < .05) were observed for 8 polymorphisms (rs1800871, rs1800872, and rs1554286 of IL-10; rs3171425 and rs7281762 of IL-10RB; rs2228048 and rs744751 of TGFBR2; and rs1800797 of IL-6) with leprosy. This association was replicated for 4 SNPs (rs1554286 of IL-10, rs7281762 of IL-10RB, rs2228048 of TGFBR2, and rs1800797 of IL-6). The interaction study revealed a significantly greater association with leprosy risk than was obtained for any SNP individually.

Conclusions: This study provides an interesting insight on the cumulative polygenic host component that regulates leprosy pathogenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Cohort Studies
  • Cytokines / genetics*
  • Cytokines / immunology*
  • Genetic Predisposition to Disease
  • Genetic Variation
  • Haplotypes
  • Host-Pathogen Interactions
  • Humans
  • India
  • Leprosy / genetics*
  • Leprosy / immunology*
  • Linkage Disequilibrium
  • Logistic Models
  • Mycobacterium leprae / immunology*
  • Polymorphism, Single Nucleotide
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Cytokines