Toward the blood-borne miRNome of human diseases

Nat Methods. 2011 Sep 4;8(10):841-3. doi: 10.1038/nmeth.1682.

Abstract

In a multicenter study, we determined the expression profiles of 863 microRNAs by array analysis of 454 blood samples from human individuals with different cancers or noncancer diseases, and validated this 'miRNome' by quantitative real-time PCR. We detected consistently deregulated profiles for all tested diseases; pathway analysis confirmed disease association of the respective microRNAs. We observed significant correlations (P = 0.004) between the genomic location of disease-associated genetic variants and deregulated microRNAs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Disease / genetics*
  • Gene Expression Profiling
  • Genetic Variation / genetics
  • Humans
  • MicroRNAs / blood*
  • MicroRNAs / genetics*
  • Oligonucleotide Array Sequence Analysis
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • MicroRNAs