Preparatory production of quercetin-3-β-D-glucopyranoside using alkali-tolerant thermostable α-L-rhamnosidase from Aspergillus terreus

Bioresour Technol. 2012 Jul:115:222-7. doi: 10.1016/j.biortech.2011.08.029. Epub 2011 Aug 11.

Abstract

Extensive screening for a robust producer of α-L-rhamnosidase activity from well-defined strains of filamentous fungi, including multifactorial optimization (inducers, cultivation conditions) was accomplished. Enzyme production of the optimal producer Aspergillus terreus (non-toxigenic) was scaled up to 50L. α-L-Rhamnosidase, which was fully characterized, proved to be thermo- and alkali-tolerant, thus enabling effective operation at 70°C and pH 8.0. These conditions allow for a very high substrate (rutin) load up to 100-300 g/L, thus enabling very high volumetric productivity of the reaction product quercetin-3-β-D-glucopyranoside (isoquercitrin). Here, a novel concept of "immobilised substrate" is used. Isoquercitrin is a highly effective and biocompatible antioxidant with strong anti-inflammatory activities. Rutin biotransformation was optimized and scaled up to ca 10 kg production and thus the robustness of the large-scale production was demonstrated. Isoquercitrin can be produced to a very high purity (98%) in multikilogram amounts, without any quercetin and directly applicable in nutraceuticals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological / drug effects*
  • Alkalies / pharmacology*
  • Aspergillus / drug effects
  • Aspergillus / enzymology*
  • Bioreactors / microbiology
  • Biotechnology / methods*
  • Biotransformation / drug effects
  • Enzyme Stability / drug effects
  • Glucosides / biosynthesis*
  • Glucosides / chemistry
  • Glycoside Hydrolases / metabolism*
  • Hydrogen-Ion Concentration / drug effects
  • Quercetin / analogs & derivatives*
  • Quercetin / biosynthesis
  • Quercetin / chemistry
  • Rutin / chemistry
  • Rutin / metabolism
  • Temperature*
  • beta-Glucosidase / metabolism

Substances

  • Alkalies
  • Glucosides
  • Rutin
  • Quercetin
  • Glycoside Hydrolases
  • beta-Glucosidase
  • alpha-L-rhamnosidase
  • quercetin 3-O-glucopyranoside