Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules

Nanoscale. 2011 Sep 1;3(9):3788-92. doi: 10.1039/c1nr10518c. Epub 2011 Aug 3.

Abstract

We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemistry
  • DNA / metabolism*
  • DNA Primers / chemistry
  • DNA-Directed DNA Polymerase / metabolism*
  • Electrodes
  • Fluorescent Dyes / chemistry
  • Gold / chemistry
  • Nanotechnology / methods*
  • Nucleic Acid Amplification Techniques / methods*

Substances

  • DNA Primers
  • Fluorescent Dyes
  • Gold
  • DNA
  • DNA-Directed DNA Polymerase