Evaluation of disk approximation and single-well broth tests for detection of inducible clindamycin resistance in Streptococcus pneumoniae

James H Jorgensen; M Leticia McElmeel; Letitia C Fulcher; Lesley McGee; Anita Glennen

doi:10.1128/JCM.00960-11

Evaluation of disk approximation and single-well broth tests for detection of inducible clindamycin resistance in Streptococcus pneumoniae

J Clin Microbiol. 2011 Sep;49(9):3332-3. doi: 10.1128/JCM.00960-11. Epub 2011 Jul 20.

Authors

James H Jorgensen¹, M Leticia McElmeel, Letitia C Fulcher, Lesley McGee, Anita Glennen

Affiliation

¹ Department of Pathology, University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, Texas 78229-3900, USA. jorgensen@uthscsa.edu

Abstract

This study evaluated an agar disk diffusion D-zone test and an erythromycin-clindamycin (ERY + CLI) single-well broth test for inducible CLI resistance in Streptococcus pneumoniae. The standard CLSI disk approximation test and a single-well combination test incorporating 1 plus 0.5 μg/ml ERY + CLI detected >96% of isolates containing the ermB determinant.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Clindamycin / pharmacology*
Drug Resistance, Bacterial*
Gene Expression Regulation, Bacterial / drug effects*
Genes, Bacterial
Humans
Microbial Sensitivity Tests / methods
Streptococcus pneumoniae / drug effects*

Substances

Anti-Bacterial Agents
Clindamycin