Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis

Proc Natl Acad Sci U S A. 2011 Jul 26;108(30):12539-44. doi: 10.1073/pnas.1103959108. Epub 2011 Jul 7.

Abstract

Jasmonate (JA) and ethylene (ET) are two major plant hormones that synergistically regulate plant development and tolerance to necrotrophic fungi. Both JA and ET induce the expression of several pathogenesis-related genes, while blocking either signaling pathway abolishes the induction of these genes by JA and ET alone or in combination. However, the molecular basis of JA/ET coaction and signaling interdependency is largely unknown. Here, we report that two Arabidopsis ET-stabilized transcription factors (EIN3 and EIL1) integrate ET and JA signaling in the regulation of gene expression, root development, and necrotrophic pathogen defense. Further studies reveal that JA enhances the transcriptional activity of EIN3/EIL1 by removal of JA-Zim domain (JAZ) proteins, which physically interact with and repress EIN3/EIL1. In addition, we find that JAZ proteins recruit an RPD3-type histone deacetylase (HDA6) as a corepressor that modulates histone acetylation, represses EIN3/EIL1-dependent transcription, and inhibits JA signaling. Our studies identify EIN3/EIL1 as a key integration node whose activation requires both JA and ET signaling, and illustrate transcriptional derepression as a common mechanism to integrate diverse signaling pathways in the regulation of plant development and defense.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism*
  • Arabidopsis / microbiology
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Base Sequence
  • Botrytis / pathogenicity
  • Cyclopentanes / metabolism*
  • DNA, Plant / genetics
  • DNA-Binding Proteins
  • Ethylenes / metabolism*
  • Histone Deacetylases / metabolism
  • Models, Biological
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Oxylipins / metabolism*
  • Plant Growth Regulators / metabolism*
  • Plant Roots / growth & development
  • Plant Roots / metabolism
  • Plants, Genetically Modified
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism
  • Signal Transduction
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Two-Hybrid System Techniques

Substances

  • Arabidopsis Proteins
  • Cyclopentanes
  • DNA, Plant
  • DNA-Binding Proteins
  • EIL1 protein, Arabidopsis
  • EIN3 protein, Arabidopsis
  • Ethylenes
  • Nuclear Proteins
  • Oxylipins
  • Plant Growth Regulators
  • Repressor Proteins
  • Transcription Factors
  • jasmonic acid
  • ethylene
  • HDA6 protein, Arabidopsis
  • Histone Deacetylases