Physiological changes in the sciatic nerve endoneurium of lead-intoxicated rats: a model of endoneurial homeostasis

Brain Res. 1990 May 28;517(1-2):1-6. doi: 10.1016/0006-8993(90)91000-7.

Abstract

The effects of inorganic lead (Pb) on peripheral nerves were studied by measuring the permeability coefficient-surface area product to albumin (PSA) of the blood-nerve interface (BNI), water and Pb content of endoneurium, and residual endoneurial plasma volume (Vp) of sciatic nerves of adult rats maintained on a 4% Pb diet for 2-12 weeks. Additionally, body weight, haematocrit, and blood Pb levels were also monitored. Within 1 week, both blood and endoneurial Pb levels had increased significantly above background levels and continued to increase up to the 6th week. Thereafter, over the next 6 weeks, the blood Pb levels were relatively stable, while the endoneurial Pb levels decreased sharply to a quarter of their peak values. The wet/dry weight ratio of the endoneurium, an indicator of nerve oedema, increased by about 30% from the 4th to 12th week, reaching a water content of 75% corresponding to a 24% increase of the total fascicular area. PSA increased only by the 8th week, without an accompanying increase in Vp. This suggests that the increase in PSA reflects an increase in the permeability of the BNI. Furthermore, the moderate 3-fold increase in PSA is more consistent with an adaptive response of the BNI to changes in the endoneurial microenvironment rather than a massive breakdown or disruption of the BNI. This is supported by the inconsistent or mild leakage of horseradish peroxidase from endoneurial capillaries observed in Pb-intoxicated rats.(ABSTRACT TRUNCATED AT 250 WORDS)

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Capillary Permeability / drug effects*
  • Connective Tissue / drug effects
  • Connective Tissue / physiopathology*
  • Edema / physiopathology*
  • Hematocrit
  • Lead / pharmacokinetics
  • Lead Poisoning / physiopathology*
  • Male
  • Peripheral Nervous System Diseases / chemically induced
  • Peripheral Nervous System Diseases / physiopathology*
  • Rats
  • Rats, Inbred Strains
  • Sciatic Nerve / blood supply
  • Sciatic Nerve / physiopathology*

Substances

  • Lead