Analysis of molluscicide metaldehyde in vegetables by dispersive solid-phase extraction and liquid chromatography-tandem mass spectrometry

Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Aug;28(8):1034-40. doi: 10.1080/19440049.2011.576439. Epub 2011 Jun 1.

Abstract

This paper describes the development and validation of a new reverse-phase liquid chromatography-electrospray ionisation tandem mass spectrometric method (RP-HPLC-ESI-MS/MS) for the determination of metaldehyde in different kinds of vegetables. Metaldehyde was extracted with 20 ml acetonitrile from 10 g vegetable and followed by dispersive solid-phase extraction (Bondesil-primary secondary amine, PSA). The identification of metaldehyde was established by chromatographic retention times, analyte-specific fragmentation patterns and relative peak area ratios of two precursor/product ion pairs. The limit of detection of this method was 0.01 mg kg(-1) using an injection volume of 5 µl. The repeatability of this method is excellent and the relative standard deviation (RSD) is less than 4%.

Publication types

  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Acetaldehyde / analogs & derivatives*
  • Acetaldehyde / analysis
  • Chromatography, High Pressure Liquid
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry / methods*
  • Vegetables / chemistry*

Substances

  • metaldehyde
  • Acetaldehyde