PERMANENT GENETIC RESOURCES: Development of microsatellite markers and analysis of their inheritance in the Australian reptile tick, Bothriocroton hydrosauri

Jaro Guzinski; Kathleen M Saint; Michael G Gardner; Stephen C Donnellan; C Michael Bull

doi:10.1111/j.1471-8286.2007.01987.x

PERMANENT GENETIC RESOURCES: Development of microsatellite markers and analysis of their inheritance in the Australian reptile tick, Bothriocroton hydrosauri

Mol Ecol Resour. 2008 Mar;8(2):443-5. doi: 10.1111/j.1471-8286.2007.01987.x.

Authors

Jaro Guzinski¹, Kathleen M Saint, Michael G Gardner, Stephen C Donnellan, C Michael Bull

Affiliation

¹ Evolutionary Biology Unit, South Australian Museum, Adelaide, SA 5000, Australia, School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia, Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, SA 5005, Australia.

PMID: 21585816
DOI: 10.1111/j.1471-8286.2007.01987.x

Abstract

Despite long-term study, the mechanism explaining the parapatric distribution of two Australian reptile tick species is not understood. We describe the development of primers amplifying 10 microsatellite Bothriocroton hydrosauri loci, for the study of population structure and dispersal patterns of this tick. The numbers of alleles per locus ranged from two to seven in ticks from the study site, and the observed heterozygosity between 0.28 and 0.69. Pedigree analysis indicates that one locus is inherited in a non-Mendelian manner in three families, which was not explained by null allele presence.