Reduced thymic output, cell cycle abnormalities, and increased apoptosis of T lymphocytes in patients with cartilage-hair hypoplasia

J Allergy Clin Immunol. 2011 Jul;128(1):139-146. doi: 10.1016/j.jaci.2011.03.042. Epub 2011 May 13.

Abstract

Background: Cartilage-hair hypoplasia (CHH) is characterized by metaphyseal dysplasia, bone marrow failure, increased risk of malignancies, and a variable degree of immunodeficiency. CHH is caused by mutations in the RNA component of the mitochondrial RNA processing (RMRP) endoribonuclease gene, which is involved in ribosomal assembly, telomere function, and cell cycle control.

Objectives: We aimed to define thymic output and characterize immune function in a cohort of patients with molecularly defined CHH with and without associated clinical immunodeficiency.

Methods: We studied the distribution of B and T lymphocytes (including recent thymic emigrants), in vitro lymphocyte proliferation, cell cycle, and apoptosis in 18 patients with CHH compared with controls.

Results: Patients with CHH have a markedly reduced number of recent thymic emigrants, and their peripheral T cells show defects in cell cycle control and display increased apoptosis, resulting in poor proliferation on activation.

Conclusion: These data confirm that RMRP mutations result in significant defects of cell-mediated immunity and provide a link between the cellular phenotype and the immunodeficiency in CHH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Apoptosis / immunology*
  • Cell Cycle / immunology*
  • Cell Separation
  • Child
  • Child, Preschool
  • Female
  • Flow Cytometry
  • Genotype
  • Hair / abnormalities
  • Hair / immunology
  • Hair / pathology
  • Hirschsprung Disease / genetics
  • Hirschsprung Disease / immunology*
  • Hirschsprung Disease / pathology
  • Humans
  • Immunologic Deficiency Syndromes / genetics
  • Immunologic Deficiency Syndromes / immunology*
  • Immunologic Deficiency Syndromes / pathology
  • Infant
  • Male
  • Mutation
  • Osteochondrodysplasias / congenital*
  • Osteochondrodysplasias / genetics
  • Osteochondrodysplasias / immunology
  • Osteochondrodysplasias / pathology
  • Phenotype
  • Polymerase Chain Reaction
  • Primary Immunodeficiency Diseases
  • RNA, Long Noncoding
  • RNA, Untranslated / genetics
  • T-Lymphocytes / immunology*
  • Thymus Gland / immunology*
  • Young Adult

Substances

  • RMRP non-coding RNA, human
  • RNA, Long Noncoding
  • RNA, Untranslated

Supplementary concepts

  • Cartilage-hair hypoplasia