Effect of cavtratin, a caveolin-1 scaffolding domain peptide, on oligodendroglial signaling cascades

Cell Mol Neurobiol. 2011 Oct;31(7):991-7. doi: 10.1007/s10571-011-9694-1. Epub 2011 Apr 27.

Abstract

Caveolin and caveolin containing rafts are involved in the signaling of growth factors in various cell types. Previous reports of our lab indicated a co-localization of caveolin and the high affinity nerve growth factor (NGF) receptor tyrosine kinase A (TrkA). Mutual effects have been observed among which a caveolin-1 knock-down resulted in an impairment of the NGF signaling cascade rather than in an increase of activity as expected from other growth factor reports. On the other hand, an over-expression of caveolin-1 impaired the NGF stimulated activity of p42/44 mitogen activated protein kinases (MAPK). In this study, we used a caveolin-1 scaffolding domain (CSD) peptide (cavtratin) of which an inhibitory effect on growth factor receptors was reported. Our data showed that cavtratin suppresses the NGF-induced phosphorylation of TrkA as well as the activation of MAPK in porcine oligodendrocytes significantly.

MeSH terms

  • Animals
  • Caveolin 1 / chemistry
  • Caveolin 1 / genetics
  • Caveolin 1 / metabolism*
  • Cells, Cultured
  • Humans
  • Mitogen-Activated Protein Kinases / metabolism
  • Nerve Growth Factor / metabolism
  • Oligodendroglia / cytology
  • Oligodendroglia / metabolism*
  • Peptides / chemistry
  • Peptides / genetics
  • Peptides / metabolism*
  • Protein Structure, Tertiary
  • Receptor, trkA / metabolism
  • Signal Transduction
  • Swine

Substances

  • Caveolin 1
  • Peptides
  • Nerve Growth Factor
  • Receptor, trkA
  • Mitogen-Activated Protein Kinases