Production of lipocortin-like proteins by cultured human tracheal submucosal gland cells

FEBS Lett. 1990 Nov 12;274(1-2):131-5. doi: 10.1016/0014-5793(90)81347-q.

Abstract

Evidence is obtained for the presence of lipocortin-like proteins in human tracheal gland cells in culture. Using polyclonal antibodies to lipocortin I, indirect immunofluorescence studies demonstrate that lipocortin I is mainly confined to the tracheal gland cell surface. From cell membranes, four Ca2(+)-dependent proteins (35, 40, 45 and 67 kDa) were identified as lipocortin related proteins by using immunoblotting and fluorography following [35S]methionine metabolic labeling experiments. A strong immunoreactivity for the 35 kDa protein was observed. In addition, lipocortin-like proteins with apparent Mr33, 35, 37 and 67 kDa, respectively, were released in the apical culture medium by tracheal gland cells cultured on microporous membrane of a double chamber culture system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Annexins
  • Calcium-Binding Proteins / biosynthesis*
  • Calcium-Binding Proteins / isolation & purification
  • Cells, Cultured
  • Electrophoresis, Polyacrylamide Gel
  • Fluorescent Antibody Technique
  • Glycoproteins / biosynthesis
  • Humans
  • Kinetics
  • Methionine / metabolism
  • Molecular Weight
  • Mucous Membrane / metabolism
  • Trachea / metabolism*

Substances

  • Annexins
  • Calcium-Binding Proteins
  • Glycoproteins
  • Methionine