Signaling molecules for early detection of adverse interactions during mechanical ventilation in animal models

In Vivo. 2011 Mar-Apr;25(2):209-17.

Abstract

Aim: The early identification of adverse interactions during mechanical ventilation, investigated by multiplexed immunoanalysis.

Materials and methods: Twenty piglets (average age 7 weeks, weight 23 kg) were intubated and divided into groups: A, spontaneously breathing; B, protectively ventilated; C, ventilated with injurious strategy; D, ventilated with lung disability. At the 1st hour (time-1) and 12th hour (time-2) of the study, brain natriuretic peptide (BNP), intercellular cell adhesion molecules (ICAM-1), vascular cell adhesion molecules (VCAM-1), tumor necrosis factor-alpha (TNF-alpha), and interleukin-6 (Il-6) were analyzed in the blood.

Results: The injurious ventilated group C exhibited an increase in both cell adhesion molecules (p<0.01), TNF-alpha and BNP (p<0.05) at time-1, and at time-2 further increases (p<0.05). In group D, an increase in ICAM-1 and BNP (p<0.05) at time-1, and increases in Il-6 and ICAM-1 (p<0.05) at time-2, with notable decreases in urine output were observed. Overall, the lung damage correlated with TNF-alpha (r=0.904), Il-6 (r=0.740), and ICAM-1 (r=0.756) levels.

Conclusion: All five monitored molecules quickly and reliably signaled adverse interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / blood*
  • Disease Models, Animal
  • Immunoassay / methods
  • Intercellular Adhesion Molecule-1 / blood
  • Interleukin-6 / blood
  • Lung / pathology
  • Lung / physiopathology
  • Lung / ultrastructure
  • Lung Injury / blood*
  • Lung Injury / diagnosis
  • Lung Injury / etiology
  • Microscopy, Electron, Transmission
  • Multiple Organ Failure / blood*
  • Multiple Organ Failure / diagnosis
  • Multiple Organ Failure / etiology
  • Natriuretic Peptide, Brain / blood
  • Respiration, Artificial / adverse effects*
  • Sensitivity and Specificity
  • Swine
  • Time Factors
  • Tumor Necrosis Factor-alpha / blood
  • Vascular Cell Adhesion Molecule-1 / blood

Substances

  • Biomarkers
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Vascular Cell Adhesion Molecule-1
  • Natriuretic Peptide, Brain
  • Intercellular Adhesion Molecule-1