Gold nanoparticles based colorimetric assay of protein poly(ADP-ribosyl)ation

Yuanyuan Xu; Jing Wang; Ya Cao; Genxi Li

doi:10.1039/c0an00806k

Gold nanoparticles based colorimetric assay of protein poly(ADP-ribosyl)ation

Analyst. 2011 May 21;136(10):2044-6. doi: 10.1039/c0an00806k. Epub 2011 Mar 30.

Authors

Yuanyuan Xu¹, Jing Wang, Ya Cao, Genxi Li

Affiliation

¹ Department of Biochemistry, National Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, PR China.

PMID: 21448503
DOI: 10.1039/c0an00806k

Abstract

A new method to assay protein poly(ADP-ribosyl)ation was proposed based on the interaction between the substrate of poly(ADP-ribosyl)ation nicotinamide adenine dinucleotide and gold nanoparticles, which needed no coupled enzymes or other modified catalytic substrate.

MeSH terms

Colorimetry / methods*
DNA / chemistry
Gold / chemistry*
Metal Nanoparticles / chemistry*
NAD / chemistry
Poly Adenosine Diphosphate Ribose / analysis*
Proteins / analysis*

Substances

Proteins
poly(ADP)-ribosylated proteins
NAD
Poly Adenosine Diphosphate Ribose
Gold
DNA