Accelerating image registration with the Johnson-Lindenstrauss lemma: application to imaging 3-D neural ultrastructure with electron microscopy

IEEE Trans Med Imaging. 2011 Jul;30(7):1427-38. doi: 10.1109/TMI.2011.2125797. Epub 2011 Mar 10.

Abstract

We present a novel algorithm to accelerate feature based registration, and demonstrate the utility of the algorithm for the alignment of large transmission electron microscopy (TEM) images to create 3-D images of neural ultrastructure. In contrast to the most similar algorithms, which achieve small computation times by truncated search, our algorithm uses a novel randomized projection to accelerate feature comparison and to enable global search. Further, we demonstrate robust estimation of nonrigid transformations with a novel probabilistic correspondence framework, that enables large TEM images to be rapidly brought into alignment, removing characteristic distortions of the tissue fixation and imaging process. We analyze the impact of randomized projections upon correspondence detection, and upon transformation accuracy, and demonstrate that accuracy is maintained. We provide experimental results that demonstrate significant reduction in computation time and successful alignment of TEM images.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Algorithms*
  • Animals
  • Databases, Factual
  • Ferrets
  • Geniculate Bodies / ultrastructure*
  • Histocytochemistry
  • Image Processing, Computer-Assisted / methods*
  • Microscopy, Electron, Transmission / methods*