Virus-poly(3,4-ethylenedioxythiophene) composite films for impedance-based biosensing

Anal Chem. 2011 Apr 1;83(7):2420-4. doi: 10.1021/ac2000835. Epub 2011 Mar 9.

Abstract

Composite films composed of poly(3,4-ethylenedioxythiophene), PEDOT, and the filamentous virus M13-K07 were prepared by electrooxidation of 3,4-ethylenedioxythiophene (EDOT) in aqueous solutions containing 8 nM of the virus at planar gold electrodes. These films were characterized using atomic force microscopy and scanning electron microscopy. The electrochemical impedance of virus-PEDOT films increases upon exposure to an antibody (p-Ab) that selectively binds to the M13 coat peptide. Exposure to p-Ab causes a shift in both real (Z(RE)) and imaginary (Z(IM)) impedance components across a broad range of frequencies from 50 Hz to 10 kHz. Within a narrower frequency range from 250 Hz to 5 kHz, the increase of the total impedance (Z(total)) with p-Ab concentration conforms to a Langmuir adsorption isotherm over the concentration range from from 6 to 66 nM, yielding a value for K(d) = 16.9 nM at 1000 Hz.

Publication types

  • Letter
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Antibodies / analysis
  • Antibodies / immunology
  • Bacteriophage M13 / chemistry*
  • Bacteriophage M13 / immunology
  • Biosensing Techniques / methods*
  • Bridged Bicyclo Compounds, Heterocyclic / chemistry*
  • Electric Impedance
  • Electroplating
  • Gold / chemistry
  • Limit of Detection
  • Polymers / chemistry*

Substances

  • Antibodies
  • Bridged Bicyclo Compounds, Heterocyclic
  • Polymers
  • poly(3,4-ethylene dioxythiophene)
  • Gold