Preparing DNA from cell pellets for genotyping

Amrik Sahota; Andrew I Brooks; Jay A Tischfield

doi:10.1101/pdb.prot4829

Preparing DNA from cell pellets for genotyping

CSH Protoc. 2007 Aug 1:2007:pdb.prot4829. doi: 10.1101/pdb.prot4829.

Authors

Amrik Sahota¹, Andrew I Brooks, Jay A Tischfield

Affiliation

¹ Rutgers University Cell and DNA Repository, Department of Genetics, Piscataway, NJ 08854-8082, USA.

PMID: 21357150
DOI: 10.1101/pdb.prot4829

Abstract

INTRODUCTIONThis protocol describes large-scale DNA extraction from cell pellets using either in-house or commercial (PUREGENE) reagents. The cell pellets are prepared from human lymphoblast cell lines (LCLs) that have been established using the Epstein-Barr virus (EBV).