Caveolae-dependent endocytosis is required for class A macrophage scavenger receptor-mediated apoptosis in macrophages

J Biol Chem. 2011 Mar 11;286(10):8231-8239. doi: 10.1074/jbc.M110.145888. Epub 2011 Jan 4.

Abstract

SR-A (class A macrophage scavenger receptor) is a transmembrane receptor that can bind many different ligands, including modified lipoproteins that are relevant to the development of vascular diseases. However, the precise endocytic pathways of SR-A/mediated ligands internalization are not fully characterized. In this study, we show that the SR-A/ligand complex can be endocytosed by both clathrin- and caveolae-dependent pathways. Internalizations of SR-A-lipoprotein (such as acLDL) complexes primarily go through clathrin-dependent endocytosis. In contrast, macrophage apoptosis triggered by SR-A-fucoidan internalization requires caveolae-dependent endocytosis. The caveolae-dependent process activates p38 kinase and JNK signaling, whereas the clathrin-mediated endocytosis elicits ERK signaling. Our results suggest that different SR-A endocytic pathways have distinct functional consequences due to the activation of different signaling cascades in macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Ulcer Agents / pharmacology
  • Apoptosis / drug effects
  • Apoptosis / physiology*
  • Caveolae / metabolism*
  • Cell Line
  • Endocytosis / physiology*
  • Enzyme Activation / drug effects
  • Enzyme Activation / physiology
  • MAP Kinase Kinase 4 / genetics
  • MAP Kinase Kinase 4 / metabolism
  • MAP Kinase Signaling System / drug effects
  • MAP Kinase Signaling System / physiology*
  • Macrophages / metabolism*
  • Mice
  • Polysaccharides / pharmacology
  • Scavenger Receptors, Class A / genetics
  • Scavenger Receptors, Class A / metabolism*
  • p38 Mitogen-Activated Protein Kinases / genetics
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Anti-Ulcer Agents
  • Polysaccharides
  • Scavenger Receptors, Class A
  • fucoidan
  • p38 Mitogen-Activated Protein Kinases
  • MAP Kinase Kinase 4