An aptamer-based chromatographic strip assay for sensitive toxin semi-quantitative detection

Biosens Bioelectron. 2011 Feb 15;26(6):3059-62. doi: 10.1016/j.bios.2010.11.040. Epub 2010 Dec 3.

Abstract

An aptamer-based chromatographic strip assay method for rapid toxin detection was developed. The aptamer-based strip assay was based on the competition for the aptamer between ochratoxin A and DNA probes. The sensing results indicated that the sensitivity of the aptamer-based strip was better than that of conventional antibody-based strips. The visual limit of detection of the strip for qualitative detection was 1 ng/mL while the LOD for semi-quantitative detection could down to 0.18 ng/mL by using scanning reader. The recoveries of test samples were from 96% to 110%. All detections could be achieved in less than 10 min, indicating that the aptamer-based strip could be a potential useful tool for rapid on-site detections.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide* / genetics
  • Base Sequence
  • Biosensing Techniques / instrumentation*
  • Biosensing Techniques / methods*
  • Biosensing Techniques / statistics & numerical data
  • DNA Probes / genetics
  • Food Contamination / analysis
  • Gold
  • Limit of Detection
  • Metal Nanoparticles
  • Microtechnology
  • Nanotechnology
  • Ochratoxins / analysis
  • SELEX Aptamer Technique / instrumentation*
  • SELEX Aptamer Technique / methods*
  • SELEX Aptamer Technique / statistics & numerical data
  • Toxins, Biological / analysis*
  • Wine / analysis

Substances

  • Aptamers, Nucleotide
  • DNA Probes
  • Ochratoxins
  • Toxins, Biological
  • ochratoxin A
  • Gold