Acetylation of EGF receptor contributes to tumor cell resistance to histone deacetylase inhibitors

Biochem Biophys Res Commun. 2011 Jan 7;404(1):68-73. doi: 10.1016/j.bbrc.2010.11.064. Epub 2010 Nov 19.

Abstract

Alteration of epidermal growth factor receptor (EGFR) is involved in various human cancers and has been intensively investigated. A plethora of evidence demonstrates that posttranslational modifications of EGFR play a pivotal role in controlling its function and metabolism. Here, we show that EGFR can be acetylated by CREB binding protein (CBP) acetyltransferase. Interestingly, EGFR acetylation affects its tyrosine phosphorylation, which may contribute to cancer cell resistance to histone deacetylase inhibitors (HDACIs). Since there is an increasing interest in using HDACIs to treat various cancers in the clinic, our current study provides insights and rationale for selecting effective therapeutic regimen. Consistent with the previous reports, we also show that HDACI combined with EGFR inhibitors achieves better therapeutic outcomes and provides a molecular rationale for the enhanced effect of combination therapy. Our results unveil a critical role of EGFR acetylation that regulates EGFR function, which may have an important clinical implication.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation / drug effects
  • Animals
  • Antineoplastic Agents / pharmacology*
  • Antineoplastic Agents / therapeutic use
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / enzymology*
  • Cell Line, Tumor
  • Cyclic AMP Response Element-Binding Protein / metabolism*
  • Drug Resistance, Neoplasm*
  • ErbB Receptors / antagonists & inhibitors
  • ErbB Receptors / genetics
  • ErbB Receptors / metabolism*
  • Female
  • Histone Deacetylase Inhibitors / pharmacology*
  • Histone Deacetylase Inhibitors / therapeutic use
  • Humans
  • Hydroxamic Acids / pharmacology
  • Hydroxamic Acids / therapeutic use
  • Lysine / genetics
  • Lysine / metabolism
  • Mice
  • Mice, Inbred Strains
  • RNA, Small Interfering / genetics
  • Vorinostat

Substances

  • Antineoplastic Agents
  • CREB1 protein, human
  • Cyclic AMP Response Element-Binding Protein
  • Histone Deacetylase Inhibitors
  • Hydroxamic Acids
  • RNA, Small Interfering
  • Vorinostat
  • ErbB Receptors
  • Lysine