Differential requirements for CD80/86-CD28 costimulation in primary and memory CD4 T cell responses to vaccinia virus

Cell Immunol. 2011;266(2):130-4. doi: 10.1016/j.cellimm.2010.09.008. Epub 2010 Oct 30.

Abstract

Vaccinia virus infection can confer immunity to smallpox by inducing potent T cell and antibody responses. While the CD8 T cell response to vaccinia virus has been well characterized, less is known about factors required for priming and memory for the CD4 T cells. Focusing on two recently described epitopes, we show that after intranasal infection, both I1L and L4R epitopes are co-dominant during the acute response, but the I1L epitope dominates during memory. CD4 T cell priming was intact in the absence of CD80/86, however secondary responses were reduced. This contrasts with our previous data showing CD80/86-CD28 interaction is required for optimal primary and memory CD8 T cell responses. The absence of CD80/86 also changed the immunodominance hierarchy during memory, with the I1L and L4R responses becoming co-dominant in knockout mice. These data highlight different costimulatory requirements for primary CD4 and CD8 T cell responses to vaccinia virus.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • B7-1 Antigen / immunology*
  • B7-2 Antigen / immunology*
  • CD28 Antigens / immunology*
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology
  • Immunologic Memory*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Vaccinia / immunology*
  • Vaccinia virus / immunology*

Substances

  • B7-1 Antigen
  • B7-2 Antigen
  • CD28 Antigens
  • Cd86 protein, mouse