The correlation among the dynamic change of Zn2+, ZnT-1, and brain-derived neurotrophic factor after acute spinal cord injury in rats

Biol Trace Elem Res. 2011 Oct;143(1):351-8. doi: 10.1007/s12011-010-8845-4. Epub 2010 Sep 14.

Abstract

Zinc plays an important role in regulating the expression of brain-derived neurotrophic factor (BDNF) and its receptor in nervous system, but the correlation among Zn(2+), zinc transporter, and BDNF in spinal cord injuries (SCI) is not fully understood. The purpose of this study was to investigate the expression of Zn(2+), zinc transporter 1 (ZnT-1), and BDNF, as well as their correlation in spinal cord-injured rats. One hundred Wistar male rats were divided into two groups: sham-operated group (as control group) and model group. Spinal cord injury was induced in model groups by hemisection of T9 on the left side. Compared with the control group, the serum zinc levels in SCI model group were significantly decreased after surgery, but zinc concentrations in spinal cord were increased gradually. The mRNA levels of ZnT-1 and BDNF were significantly increased in SCI model group, and there is a positive correlation between them (Spearman rho = 0.381, P = 0.0204). The correlation found between BDNF and ZnT-1 allows us to speculate that these two factors may be physiologically co-regulated, which may provide an idea for the treatment of SCI.

MeSH terms

  • Animals
  • Brain-Derived Neurotrophic Factor / genetics*
  • Cation Transport Proteins / genetics*
  • Male
  • Rats
  • Rats, Wistar
  • Real-Time Polymerase Chain Reaction
  • Spinal Cord Injuries / blood*
  • Spinal Cord Injuries / genetics*
  • Zinc / blood*

Substances

  • Brain-Derived Neurotrophic Factor
  • Cation Transport Proteins
  • Slc30a1 protein, rat
  • Zinc