Flavonoids as inhibitors of rat liver cytosolic glutathione S-transferase

Abstract

The inhibitory potencies of different flavonoids for rat liver cytosolic glutathione S-transferase activity varied over 30-fold, depending on the pattern of hydroxylation, the presence of a C-2, C-3 double bond and the substitution of a hydroxyl group with a sugar moiety. Kinetic inactivation studies of the enzyme with the inhibitor quercetin revealed a non-competitive profile versus both glutathione and 1-chloro-2,4-dinitrobenzene.