There is an increasing demand for composite resins with both strong antibacterial activity and satisfactory mechanical properties. This study tested the hypothesis that the new antibacterial agent tetrapod-like zinc oxide whisker (T-ZnOw) could simultaneously enhance the antibacterial activity and mechanical properties of a two-component composite resin. The antibacterial activities of the materials were assessed by the broth dilution test and direct contact test. Optical microscopy, scanning electron microscopy, and measurements of the flexural strength, compressive strength, and diametral tensile strength were carried out for mechanical characterization. The results revealed that T-ZnOw provided the resin with strong antibacterial activity and improved mechanical properties in all tested groups. However, the antibacterial activity of the resin with 10% T-ZnOw in the powder component significantly decreased after aging treatment. The incorporation of 5% T-ZnOw into the resin powder was optimal to give appropriate antibacterial activity, long-term antibacterial effectiveness, and mechanical properties.