Isolation of intact genomic DNA from FOXP3-sorted human regulatory T cells for epigenetic analyses

Eur J Immunol. 2010 May;40(5):1510-2. doi: 10.1002/eji.200940154.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cell Lineage
  • Cell Separation / methods*
  • CpG Islands
  • DNA / genetics
  • DNA / isolation & purification*
  • DNA / metabolism
  • DNA Methylation
  • Epigenesis, Genetic*
  • Flow Cytometry / methods*
  • Forkhead Transcription Factors / metabolism*
  • Genome, Human
  • Humans
  • Molecular Weight
  • Protein Binding
  • Specimen Handling
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Staining and Labeling
  • T-Lymphocyte Subsets / chemistry*
  • T-Lymphocyte Subsets / metabolism
  • T-Lymphocytes, Regulatory / chemistry*
  • T-Lymphocytes, Regulatory / metabolism
  • Tissue Fixation

Substances

  • FOXP3 protein, human
  • Forkhead Transcription Factors
  • DNA