A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae

Monica Imperi; Marco Pataracchia; Giovanna Alfarone; Lucilla Baldassarri; Graziella Orefici; Roberta Creti

doi:10.1016/j.mimet.2009.11.010

A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae

J Microbiol Methods. 2010 Feb;80(2):212-4. doi: 10.1016/j.mimet.2009.11.010. Epub 2009 Dec 1.

Authors

Monica Imperi¹, Marco Pataracchia, Giovanna Alfarone, Lucilla Baldassarri, Graziella Orefici, Roberta Creti

Affiliation

¹ Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, 00161 Rome, Italy.

PMID: 19958797
DOI: 10.1016/j.mimet.2009.11.010

Abstract

A multiplex PCR assay for the identification of serotypes Ia to IX of Streptococcus agalactiae was developed. By using a single PCR reaction containing a mix of 19 primers the assay identified each serotype by the analysis of the unique two or three bands pattern on agarose gel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Capsules / classification*
Bacterial Capsules / genetics
Bacterial Typing Techniques / methods*
DNA Primers / genetics
DNA, Bacterial / genetics*
Electrophoresis, Agar Gel
Humans
Polymerase Chain Reaction / methods*
Streptococcus agalactiae / classification*
Streptococcus agalactiae / genetics

Substances

DNA Primers
DNA, Bacterial