Kinetics of apatite formation on a calcium-silicate cement for root-end filling during ageing in physiological-like phosphate solutions

Clin Oral Investig. 2010 Dec;14(6):659-68. doi: 10.1007/s00784-009-0356-3. Epub 2009 Nov 27.

Abstract

The bioactivity of calcium silicate mineral trioxide aggregate (MTA) cements has been attributed to their ability to produce apatite in presence of phosphate-containing fluids. This study evaluated surface morphology and chemical transformations of an experimental accelerated calcium-silicate cement as a function of soaking time in different phosphate-containing solutions. Cement discs were immersed in Dulbecco's phosphate-buffered saline (DPBS) or Hank's balanced salt solution (HBSS) for different times (1-180 days) and analysed by scanning electron microscopy connected with an energy dispersive X-ray analysis (SEM-EDX) and micro-Raman spectroscopy. SEM-EDX revealed Ca and P peaks after 14 days in DPBS. A thin Ca- and P-rich crystalline coating layer was detected after 60 days. A thicker multilayered coating was observed after 180 days. Micro-Raman disclosed the 965-cm(-1) phosphate band at 7 days only on samples stored in DPBS and later the 590- and 435-cm(-1) phosphate bands. After 60-180 days, a layer approximately 200-900 μm thick formed displaying the bands of carbonated apatite (at 1,077, 965, 590, 435 cm(-1)) and calcite (at 1,088, 713, 280 cm(-1)). On HBSS-soaked, only calcite bands were observed until 90 days, and just after 180 days, a thin apatite-calcite layer appeared. Micro-Raman and SEM-EDX demonstrated the mineralization induction capacity of calcium-silicate cements (MTAs and Portland cements) with the formation of apatite after 7 days in DPBS. Longer time is necessary to observe bioactivity when cements are immersed in HBSS.

MeSH terms

  • Aluminum Compounds / chemistry*
  • Apatites / chemistry*
  • Biocompatible Materials / chemistry*
  • Buffers
  • Calcium / analysis
  • Calcium Carbonate / chemistry
  • Calcium Compounds / chemistry*
  • Chemical Precipitation
  • Crystallization
  • Drug Combinations
  • Electron Probe Microanalysis
  • Humans
  • Hydrogen-Ion Concentration
  • Isotonic Solutions / chemistry
  • Kinetics
  • Materials Testing
  • Microscopy, Electron, Scanning
  • Microspectrophotometry
  • Oxides / chemistry*
  • Phosphates / chemistry*
  • Phosphorus / analysis
  • Porosity
  • Retrograde Obturation
  • Root Canal Filling Materials / chemistry*
  • Silicates / chemistry*
  • Sodium Chloride / chemistry
  • Spectrum Analysis, Raman
  • Surface Properties
  • Temperature
  • Time Factors

Substances

  • Aluminum Compounds
  • Apatites
  • Biocompatible Materials
  • Buffers
  • Calcium Compounds
  • Drug Combinations
  • Hanks Balanced Salt Solution
  • Isotonic Solutions
  • Oxides
  • Phosphates
  • Root Canal Filling Materials
  • Silicates
  • mineral trioxide aggregate
  • Phosphorus
  • Sodium Chloride
  • carboapatite
  • Calcium Carbonate
  • calcium silicate
  • Calcium