Fibrin clot properties in acute ischemic stroke: relation to neurological deficit

Thromb Res. 2010 Apr;125(4):357-61. doi: 10.1016/j.thromres.2009.11.013. Epub 2009 Nov 26.

Abstract

Introduction: Hypercoagulable state occurs in patients with acute vascular events. We wondered whether clot structure/function is altered in acute ischemic stroke (AIS), like in acute myocardial infarction.

Patients and methods: In 45 consecutive patients with AIS (24M, 21F), aged 67.4+/-10.9 years, and 45 healthy controls matched for age and sex, we investigated plasma fibrin clot structure/function by permeation, turbidity, and efficiency of fibrinolysis.

Results: Compared to controls, AIS patients produced clots that had 30.5% less porous network (p<0.0001), were less susceptible to fibrinolysis (10.8% longer lysis time, p=0.001), were 20.5% more compact (p<0.0001), had 17.1% higher clot mass (p<0.0001), and showed increased (by 10.2%) overall fiber thickness (p<0.0001) with 8% shorter lag phase of fibrin formation (p=0.0002). Maximum rate of D-dimer release from clots was similar. Multiple regression analyses for all subjects (n=90) showed that being a stroke patient (p<0.0001), fibrinogen (p<0.0001) and lipoprotein(a) (p=0.0075) were independent predictors of clot permeability (model R2 0.79). Only fibrinogen (p<0.0001) and lipoprotein(a) (p=0.0026) predicted lysis time. All other fibrin parameters were predicted only by being a stroke patient. Clot compaction was associated with neurological deficit on admission (r=-0.81; p<0.0001) and at discharge (r=-0.69; p<0.0001). Patients with 0 or 1 point in the modified Rankin scale (n=19) had 13.3% higher clot permeability compared to the remainder (p=0.02).

Conclusions: This study is the first to show that AIS is associated with unfavorably altered fibrin clot properties that might correlate with neurological deficit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Aged
  • Blood Coagulation Tests
  • Family
  • Female
  • Fibrin / metabolism*
  • Fibrin Fibrinogen Degradation Products / metabolism
  • Fibrinogen / genetics
  • Fibrinolysis / physiology*
  • Humans
  • Intracranial Thrombosis
  • Ischemia
  • Male
  • Middle Aged
  • Permeability
  • Stroke / blood*
  • Thrombosis / blood

Substances

  • Fibrin Fibrinogen Degradation Products
  • fibrin fragment D
  • Fibrin
  • Fibrinogen