JAK2V617F mutation in patients with splanchnic vein thrombosis

Dig Dis Sci. 2010 Jun;55(6):1770-7. doi: 10.1007/s10620-009-0933-y. Epub 2009 Aug 19.

Abstract

Background: Splanchnic vein thrombosis can be the presenting manifestation of myeloproliferative neoplasms. However, the diagnosis of a myeloproliferative neoplasm in these patients is often problematic, and more objective criteria are needed.

Aim: To determine the frequency of the mutation JAK2V617F in patients with splanchnic vein thromboses.

Methods: A consecutive series of 108 adult patients with portal vein thrombosis (n = 77) and Budd-Chiari syndrome (n = 31) referred for hemostasis evaluation was retrospectively studied, with a median follow-up of 51 months (1-104).

Results: One or more prothrombotic risk factors were present in 63% of the patients. Twenty-four (22%) out of the 108 patients presented the JAK2V617F, including 2 cirrhotic patients. Most had a low mutated allele burden (median 16.5%). JAK2V617F was present in all four patients with a previous diagnosis of a myeloproliferative neoplasm. In nine JAK2V617F-positive patients, the diagnosis of a myeloproliferative neoplasm was made at the thrombosis work-up, during follow-up or after JAK2V617F detection. Among the other 11 patients carrying the mutation, 2 patients have died, 4 had no evidence suggesting a myeloproliferative neoplasm, 1 had a normal bone marrow biopsy, and the other 4 could not be persuaded to undergo a biopsy. Among the patients without an overt myeloproliferative neoplasm, 15 out of 99 (15%) presented the JAK2V617F mutation. None of the JAK2V617F-negative patients have developed signs of a myeloproliferative neoplasm during follow-up.

Conclusions: Our findings suggest that JAK2V617F occurs in a high proportion of patients with splanchnic vein thrombosis, and reinforces the diagnostic utility of JAK2V617F testing in this setting.

Publication types

  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Brazil
  • Budd-Chiari Syndrome / diagnosis
  • Budd-Chiari Syndrome / enzymology
  • Budd-Chiari Syndrome / genetics*
  • Budd-Chiari Syndrome / physiopathology
  • Chi-Square Distribution
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Genetic Testing
  • Humans
  • Janus Kinase 2 / genetics*
  • Male
  • Middle Aged
  • Mutation*
  • Myeloproliferative Disorders / diagnosis
  • Myeloproliferative Disorders / enzymology
  • Myeloproliferative Disorders / genetics*
  • Myeloproliferative Disorders / physiopathology
  • Phenotype
  • Portal Vein* / physiopathology
  • Predictive Value of Tests
  • Retrospective Studies
  • Risk Assessment
  • Risk Factors
  • Splanchnic Circulation / genetics
  • Time Factors
  • Venous Thrombosis / diagnosis
  • Venous Thrombosis / enzymology
  • Venous Thrombosis / genetics*
  • Venous Thrombosis / physiopathology
  • Young Adult

Substances

  • JAK2 protein, human
  • Janus Kinase 2