Double-blind crossover study of the cognitive effects of lorazepam in healthy apolipoprotein E (APOE)-epsilon4 carriers

J Clin Psychiatry. 2009 Oct;70(10):1379-84. doi: 10.4088/JCP.08m04593. Epub 2009 Jun 30.

Abstract

Objective: To examine cognitive effects of pharmacologically induced somnolence in cognitively normal carriers and noncarriers of the apolipoprotein E (APOE)-epsilon4 allele, a common Alzheimer's disease susceptibility gene.

Method: Between December 2005 and July 2007, healthy and cognitively normal carriers of the APOE-epsilon4 allele (heterozygotes; n = 18) and noncarriers (n = 18), 50 to 65 years old, participated in a double-blind crossover study of cognitive function before, 2.5 hours after, and 5 hours after administration of 2 mg oral lorazepam or placebo. Main outcome measures included the Groton Maze Learning Test (GMLT) for executive functioning and visuospatial working memory, the Rey Auditory-Verbal Learning Test (AVLT) for verbal memory, and the one-back test for attention and simple working memory.

Results: At 2.5 hours after lorazepam administration, GMLT total errors score (P = .04), AVLT long-term memory (P = .01), and AVLT percent recall (P = .005) reflected worse performance in heterozygotes. By multivariate analysis, the combined set of all 6 measures for heterozygotes versus noncarriers yielded P = .003 for 2.5 hours and P = .58 for 5 hours. No differences were observed for somnolence, speed, attention, or simple working memory at any time points.

Conclusions: Despite comparable levels of associated somnolence, lorazepam appears to diminish verbal and visuospatial memory more in healthy late-middle-aged heterozygotes than in noncarriers, whereas attention and reaction time are similarly affected in both. Additional studies are needed to determine whether substantial lorazepam-induced memory detriments predict subsequent onset of cognitive decline and conversion to mild cognitive impairment or Alzheimer's disease. Clinicians should be aware of the potential for cognitive decline with lorazepam in healthy late-middle-aged individuals, especially those at a higher risk for Alzheimer's disease.

Trial registration: clinicaltrials.gov Identifier: NCT00586430.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Alleles
  • Alzheimer Disease / chemically induced
  • Alzheimer Disease / etiology
  • Apolipoprotein E4 / genetics*
  • Cognition / drug effects*
  • Cognition Disorders / chemically induced*
  • Cross-Over Studies
  • Dose-Response Relationship, Drug
  • Double-Blind Method
  • Female
  • Genetic Predisposition to Disease / genetics
  • Heterozygote*
  • Homozygote
  • Humans
  • Hypnotics and Sedatives / pharmacology*
  • Lorazepam / pharmacology*
  • Male
  • Maze Learning / drug effects
  • Memory / drug effects
  • Memory Disorders / chemically induced
  • Middle Aged
  • Neuropsychological Tests / statistics & numerical data
  • Verbal Learning / drug effects

Substances

  • Apolipoprotein E4
  • Hypnotics and Sedatives
  • Lorazepam

Associated data

  • ClinicalTrials.gov/NCT00586430