Enabling IMAC purification of low abundance recombinant proteins from E. coli lysates

Nat Methods. 2009 Jul;6(7):477-8. doi: 10.1038/nmeth0709-477.
No abstract available

Publication types

  • Letter

MeSH terms

  • Chelating Agents
  • Chromatography, Affinity / methods*
  • Escherichia coli / chemistry*
  • Escherichia coli / genetics*
  • Green Fluorescent Proteins / chemistry
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / isolation & purification
  • Histidine / genetics
  • Molecular Weight
  • Nickel
  • Oligopeptides / genetics
  • Osmotic Pressure
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics*
  • Recombinant Proteins / isolation & purification*

Substances

  • Chelating Agents
  • His-His-His-His-His-His
  • Oligopeptides
  • Recombinant Proteins
  • Green Fluorescent Proteins
  • Histidine
  • Nickel