An analytical method incorporating simple liquid extraction followed by mixed mode cation exchange/reversed phase solid phase extraction and liquid chromatography-tandem mass spectrometry was developed and validated for the analysis of melamine (MEL) in liquid and powdered infant formula. The method used two different MEL stable isotope labeled internal standards to monitor analyte recoveries and to account for matrix effects. The method is sensitive (limit of quantitation of 4 ng/g), accurate, and precise (during validation, recoveries corrected by internal recovery standard averaged between 92 and 104% for all fortification levels and matrices). The method was used to analyze 94 samples of infant formula purchased from major retailers in Ottawa, ON, Canada, to examine whether or not Canadian infants are exposed to background levels of MEL. MEL was detected in 71 of the 94 products analyzed at concentrations ranging from 4.31 to 346 ng/g (median = 16 ng/g). A comparison of estimated dietary exposures to the recently recommended World Health Organization toxicological reference value for melamine suggests that the presence of low levels of MEL in infant formula purchased in Canada does not represent a health risk.