Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA

Science. 1991 Oct 25;254(5031):576-8. doi: 10.1126/science.1948034.

Abstract

A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and gamma-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT ([3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane) to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / drug effects
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Line
  • Cell Membrane / metabolism
  • Cloning, Molecular
  • Cocaine / analogs & derivatives
  • Cocaine / metabolism
  • Cocaine / pharmacology*
  • Dopamine / metabolism*
  • Dopamine Plasma Membrane Transport Proteins
  • Female
  • Kinetics
  • Membrane Glycoproteins*
  • Membrane Transport Proteins*
  • Models, Structural
  • Molecular Sequence Data
  • Nerve Tissue Proteins*
  • Oligodeoxyribonucleotides
  • Oocytes / physiology
  • Plasmids
  • Polymerase Chain Reaction
  • Protein Conformation
  • RNA, Messenger / genetics
  • Rats
  • Transcription, Genetic
  • Transfection
  • Xenopus

Substances

  • Carrier Proteins
  • Dopamine Plasma Membrane Transport Proteins
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Nerve Tissue Proteins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • (1R-(exo,exo))-3-(4-fluorophenyl)-8-methyl-8- azabicyclo(3.2.1)octane-2-carboxylic acid, methyl ester
  • Cocaine
  • Dopamine

Associated data

  • GENBANK/M76239
  • GENBANK/M76240
  • GENBANK/M76241
  • GENBANK/M76242
  • GENBANK/M76243
  • GENBANK/M76244
  • GENBANK/M80570
  • GENBANK/M83741
  • GENBANK/M83742
  • GENBANK/S70464